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一般說明
调整ThermalSealRTS™膜的尺寸以使其适合于边缘抬升的96孔板的边缘。这些膜具有始终如一的高光学清晰度，使得可重复、可靠且一致的DNA扩增测量和晶体检测成为可能。两个端部凸片有助于将薄膜定位在板上，并且非粘性的粘合表面简化了处理。轻松去除穿孔边界处的端部突片可以防止提起和与板边缘重叠的薄膜蒸发速率增大。
尺寸 76.2 x 133.4 mm。移除末端翼片后，长度为 113.0 mm。
- 高光学清晰度
- 极少至无自发荧光
- 化学惰性；除在极端 pH 值下外，无浸提物
- 耐 HTS 的 DMSO
- 耐热，建议温度范围为
- -70°C至+100°C
- 经认证无DNA酶、RNA酶和核酸
- 安装在凸起的板缘内，以防止因薄膜抬起而导致密封损失
- 硅酮胶形成最坚固的密封，可防止蒸发
- 非粘性粘合剂层可简化密封前的薄膜处理
法律資訊
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实验方案
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
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