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Merck
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主要文件

Z374911

PCR 多孔板

size 384 wells, polypropylene, skirt, non-sterile

别名:

384 multiwell PCR plate, 384 well PCR microplate, 384 well PCR plate, 384 well microtiter plate

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About This Item

分類程式碼代碼:
41106300
NACRES:
NB.22

材料

colorless polypropylene
polypropylene

無菌

non-sterile
non-sterile

特點

skirt

包裝

case of 50 ea

製造商／商標名

Sorenson 39620

技術

PCR: suitable

尺寸

384 wells

孔容積

40 μL

孔有效容積

25 μL

適合性

suitable for (PCR, RT-PCR or DNA purification applications)

應用

agriculture

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相关类别

一般說明

刚性顶板（随附）可最大限度地减小板变形，确保与PCR热循环仪的可靠配合，并允许使用微垫子或帽条进行防漏密封。每孔最大容量为200μL。
384 well PCR plates are skirted for compatibility with automation systems. Wells have raised rims to ensure contact with sealing film and reduce evaporation. Each well has a capacity of 40 μL and a working volume of 25 μL.

特點和優勢

  • 原始聚丙烯
  • 完全可高压灭菌
  • 经认证的无DNasa和RNase
  • 微孔壁薄，可实现快速温度平衡并缩短周期

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Sigma-Aldrich

Z711063

Greiner 96 well plates, polypropylene

实验方案

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

Primer Concentration Optimization Protocol

Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

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