推荐产品
生物源
human
重組細胞
expressed in HEK 293 cells
化驗
≥97% (HPLC)
≥97% (SDS-PAGE)
形狀
lyophilized
分子量
60.0-65.0 kDa
包裝
pkg of 50 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
適合性
suitable for molecular biology
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... SPP1(6696)
一般說明
Research area: Cell signaling. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, and odontoblasts. Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0kDa by SDS-PAGE analysis under reducing conditions.
應用
Osteopontin human has been used in in vitro invasion assay to study the effect of endostar on non-small cell lung cancer cell invasion.It has been used in the cell culture of primary human brain vascularsmooth muscle cells. Osteopontin human has also been used to study itseffects on the migration of human fibroblasts.
生化／生理作用
Osteopontin is a secreted glycoprotein that functions as a ligand to αvβ3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNg (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin is also associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and suppresses ectopic calcification.
序列
IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN
外觀
Lyophilized from 10 mM Sodium Phosphate, pH 7.0.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel.
Boskey AL, et al.
Bone and Mineral, 22, 147-159 (1993)
Endostar, a modified endostatin inhibits non small cell lung cancer cell in vitro invasion through osteopontin-related mechanism.
Ni Q, et al.
European Journal of Pharmacology, 614, 1-6 (2009)
W T Butler
Connective tissue research, 23(2-3), 123-136 (1989-01-01)
Osteopontin is an acidic glycoprotein of about 41,500 daltons that has been isolated from rat, human and bovine bone. It is rich in aspartic acid, glutamic acid and serine and contains about 30 monosaccharides, including 10 sialic acids. Several types
D T Denhardt et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 7(15), 1475-1482 (1993-12-01)
In this review most of the various known, suspected, or postulated functions of osteopontin, a secreted highly acidic phosphoprotein, are discussed in terms of what we currently know about the protein. These include 1) binding of OPN both to cells
Post-translational modification and proteolytic processing of urinary osteopontin.
Christensen B, et al.
The Biochemical Journal, 411, 53-61 (2008)
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