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生物源
human
重組細胞
expressed in E. coli
化驗
≥70% (SDS-PAGE)
形狀
aqueous solution
分子量
59 kDa
包裝
pkg of 100 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
>0.02 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... SIRT3(23410)
一般說明
Sirtuin 3 (SIRT3) is a member of a family of NAD+-dependent protein deacetylases called sirtuins (SIRTs). In mammals, there are seven types of SIRTs (SIRT1 to 7). SIRT 3 is present in mitochondrial matrix.
應用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化／生理作用
Sirtuin 3 (SIRT3) is thought to be involved in metabolism as its expression is suppressed in muscles of diabetic animals and increased in white and brown adipose tissues during calorie restriction (CR). This protein modulates the degree of acetylation and activity of acetyl-coenzyme A synthetase 2, an enzyme thought to be involved in energy production during starvation conditions in mammals. Up-regulation of this gene influences the expression of genes involved in mitochondrial function. During fasting, SIRT3 controls mitochondrial intermediary metabolism and fatty-acid use by deacetylating long-chain acyl coenzyme A dehydrogenase (LCAD). It is an essential modulator of basal ATP, and therefore, overall energy homeostasis.
單位定義
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation.
Hirschey MD, et al.
Nature, 464(7285), 121-125 (2010)
A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis.
Ahn BH
Proceedings of the National Academy of Sciences of the USA, 105(38), 14447-14452 (2008)
David B Lombard et al.
Molecular and cellular biology, 27(24), 8807-8814 (2007-10-10)
Homologs of the Saccharomyces cerevisiae Sir2 protein, sirtuins, promote longevity in many organisms. Studies of the sirtuin SIRT3 have so far been limited to cell culture systems. Here, we investigate the localization and function of SIRT3 in vivo. We show
L Pacella-Ince et al.
Human reproduction (Oxford, England), 29(7), 1490-1499 (2014-04-29)
Is the activity of sirtuin 3 (SIRT3) altered in granulosa and cumulus cells from young women with reduced ovarian reserve or women of advanced maternal age? SIRT3 mRNA and active protein in granulosa and cumulus cells were decreased in women
Yang Cui et al.
PloS one, 10(6), e0129834-e0129834 (2015-06-30)
SIRT3 is a key NAD+-dependent protein deacetylase in the mitochondria of mammalian cells, functioning to prevent cell aging and transformation via regulation of mitochondrial metabolic homeostasis. However, SIRT3 is also found to express in some human tumors; its role in
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