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Key Documents

SRP0112

Sigma-Aldrich

HDAC-10 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

别名:

HD10, Histone deacetylase 10

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥10% (SDS-PAGE)

形狀

aqueous solution

分子量

79 kDa

包裝

pkg of 50 μg

儲存條件

avoid repeated freeze/thaw cycles

濃度

>0.02 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... HDAC10(83933)

一般說明

Human HDAC10, amino acids 1-481, GenBank Accession No. NM_032019, with N-terminal GST tag, MW = 79 kDa, expressed in baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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