推荐产品
應用
萤火虫荧光素酶在分子和细胞生物学中广泛使用,特别是用于 ATP 的有效检测和定量,并作为遗传功能的报告基因。
生化/生理作用
萤火虫荧光素酶是一种 62 kDa 的蛋白质,催化光的产生。这种酶需要 ATP、分子氧和杂环化合物萤火虫荧光素,在两步过程中产生光。光产生反应由荧光素活化(其羧基的腺苷酸化)启动,并在分子氧存在下进行,产生黄绿色光光子。
單位定義
一个荧光素酶单位在 20-25℃ 下将在10 秒内产生一个相对光单位 (RLU),使用 GloMax 20/20 光度计在Tris-甘氨酸缓冲液中含有40pmol ATP和15nmol荧光素的100μl测定混合物中测定,溶液PH值为7.6。
单位定义换算系数:每个传统照明单位大约有 9000 个相对照明单位 (RLU),在PPO / POPOP混合物中使用相当于0.02μ Ci 14 C 的峰高。
单位定义换算系数:每个传统照明单位大约有 9000 个相对照明单位 (RLU),在PPO / POPOP混合物中使用相当于0.02μ Ci 14 C 的峰高。
外觀
以含有 HEPES (pH 7.5)、NaCl、MgCl 2 、EDTA、DTT 的冻干粉和碳水化合物稳定剂形式提供。
本品是由 大肠杆菌表达的 luc 基因产生的来自 Photinus pyralis(美国萤火虫)的重组荧光素酶 (62 kDa) 。
準備報告
为了获得最大溶解度,重要的是在高盐浓度下复溶酶,如 1 MTris 缓冲液与 pH 7-8 的任何反离子。制备的酶浓度每毫升可高达 5 mg 蛋白。切勿涡旋,避免搅拌。
复溶后,酶溶液可在 4-8°C 下保存长达 2 天,或在-20°C 下以工作等份冷冻保存至少一个月。不推荐反复冻融。
复溶后,酶溶液可在 4-8°C 下保存长达 2 天,或在-20°C 下以工作等份冷冻保存至少一个月。不推荐反复冻融。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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商品
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
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