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F4759

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, powder, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

EC號碼:
289-149-2
MDL號碼:
MFCD00131062
分類程式碼代碼:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

纤连蛋白牛血浆, powder, BioReagent, suitable for cell culture

生物源

bovine plasma

品質等級

200

無菌

sterile

產品線

BioReagent

形狀

powder

分子量

~450 kDa

包裝

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

濃度

≥1 mg protein/mL

技術

cell culture | mammalian: suitable

表面覆盖率

1‑5 μg/cm2

溶解度

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI登錄號

NM_001163778.1

UniProt登錄號

P07589

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

bovine ... FN1(2807940)

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相关类别

一般說明

纤连蛋白是一种具有多个结构域的糖蛋白。它包含一个N端、中央结合域和一个肝素结合域。牛血浆纤连蛋白具有带二硫键的多肽链，分子量分别为220和215 kDa。
纤连蛋白是粘着糖蛋白，存在于细胞表面和血浆中。纤连蛋白（FN）是一种多功能糖蛋白，相应的基因定位于人染色体2q34-36。该基因有75kb长并含有50个外显子。它存在有两种主要的异构体，一个可溶而另一种不可溶。前者存在与血浆中，而不可溶形式则存在于组织和软骨的细胞外基质（ECM）中。因在不同的区域存在可变剪切，故存在约20种不同的FN形式，其中有两个III型外显子的被称为Extra Domains A和B。

應用

来自于牛血浆的纤连蛋白可用于以下应用：
  • 用于涂布玻璃盖玻片以用于蜕膜基质细胞（DEC）的培养
  • 细胞运动检测（培养板涂有纤连蛋白）
  • 细胞扩散和免疫荧光
  • 蛋白质特异性
  • 将传感器膜集成到微流控芯片中
  • 组织学和免疫组织化学（IHC）分析
  • 用于来自肺癌患者的原代细胞培养

生化／生理作用

纤连蛋白是蛋白质支架的一种调节因子。它协调细胞过程，如血小板功能、伤口愈合、粘附、迁移和聚集。研究表明人牙髓 (HDP) 细胞依靠纤连蛋白 (FN)实现最初的附着和之后的扩散。FN可能具有促有丝分裂活性，促进细胞增殖，也在髓细胞向成牙质细胞的分化过程的调节中起一定作用。FN 的存在影响 HDP细胞的发展和命运，特别是在分化为成牙质细胞的过程中，成牙质细胞可形成牙齿中的牙本质。

注意

供应后，该产品应当储存在-20°C并将保持活性至少两年。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft.
Cigdem Aydin et al.
Human gene therapy, 18(1), 39-50 (2006-12-26)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not
Toru Yoshihara et al.
PLoS genetics, 14(8), e1007545-e1007545 (2018-08-17)
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using
Wagner Shin Nishitani et al.
PloS one, 10(5), e0126440-e0126440 (2015-05-07)
A cell mechanical stimulation equipment, based on cell substrate deformation, and a more sensitive method for measuring adhesion of cells were developed. A probe, precisely positioned close to the cell, was capable of a vertical localized mechanical stimulation with a
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
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