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應用
来自费氏弧菌的荧光素酶已用于一项评估生物发光细菌的光发射和氧消耗动力学的研究中。它也被用于一项调查各种发光细菌的暗突变体对活性氧物种敏感性的研究中。
特點和優勢
部分纯化的、含有FMN依赖性荧光素酶和NADH-和NADPH依赖性FMN还原酶的可溶性提取物。在含有FMN、NADH或NADPH和正癸基醛的体系中产生光。
其他說明
ATCC No. 7744平衡主要的缓冲液和稳定剂。
外觀
部分纯化的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Patricia G Cruz et al.
Chemistry & biology, 18(11), 1442-1452 (2011-11-29)
The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in
J J Bourgois et al.
Journal of bioenergetics and biomembranes, 33(4), 353-363 (2001-11-17)
Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with
Anya Bakhrat et al.
Cell biology and toxicology, 27(3), 227-236 (2011-03-03)
We describe a Saccharomyces cerevisiae bioluminescence assay for UV and arsenate in which bacterial luciferase genes are regulated by the promoter of the yeast gene, UFO1. UFO1 encodes the F-box subunit of the Skp1–Cdc53–F-box protein ubiquitin ligase complex and is
Ismael Rodea-Palomares et al.
Archives of environmental contamination and toxicology, 57(3), 477-487 (2009-01-27)
The bioavailability and therefore toxicity of a metal depends on the chemical species present in a particular environment. We evaluated the effect of a series of factors that could potentially modify metal speciation on the toxicity of Hg, Cu, Zn
M-L Foucault et al.
Applied and environmental microbiology, 76(1), 264-274 (2009-11-03)
Bioluminescence imaging (BLI) is emerging as a powerful tool for real-time monitoring of infections in living animals. However, since luciferases are oxygenases, it has been suggested that the requirement for oxygen may limit the use of BLI in anaerobic environments
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