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Merck

R1383

Sigma-Aldrich

RPMI-1640 培养基

With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

别名:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

RPMI-1640 培养基, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

形狀

powder

技術

cell culture | mammalian: suitable

成分

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

應用

在各种细胞培养实验中,RPMI-1640 培养基已被用作培养人MCF-7乳腺癌细胞 和人去势抵抗性前列腺癌 (CRPC) 细胞系的细胞培养基。它还被用于培养人胰岛,以评估原代人胰岛中的氧化应激反应。

數量

配制成每升培养基中含 8.4 克粉末。

重構

补充2.0 g/L碳酸氢钠。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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