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product name
RPMI-1640 培养基, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture
形狀
powder
技術
cell culture | mammalian: suitable
成分
L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
應用
在各种细胞培养实验中,RPMI-1640 培养基已被用作培养人MCF-7乳腺癌细胞 和人去势抵抗性前列腺癌 (CRPC) 细胞系的细胞培养基。它还被用于培养人胰岛,以评估原代人胰岛中的氧化应激反应。
數量
配制成每升培养基中含 8.4 克粉末。
重構
补充2.0 g/L碳酸氢钠。
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产品编号
说明
价格
補充物
产品编号
说明
价格
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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