品質等級
形狀
powder
技術
cell culture | mammalian: suitable
成分
phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员,可以根据应用灵活优化配方。
杜氏改良Eagle培养基(DMEM)是对Eagle基础培养基 (BME)的改良,含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。
應用
杜氏改良Eagle′培养基已用于:
- 培养分离的指短屈肌(FDB)纤维,以测量外源脂肪酸(FA)的利用率,作为耗氧率(OCR)测量的一部分
- 培养离体胫骨前肌纤维以测定乳酸含量
- 培养用于成骨分化的人骨髓间充质干细胞,制备培养人肝细胞癌HepG2细胞的低糖培养基
數量
按照每升培养基8.3克粉末配制。
重構
补充1.0g/L葡萄糖,0.584g/L L-谷氨酰胺,3.7g/L碳酸氢钠。
也與該產品經常一起購買
产品编号
说明
价格
補充物
产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
Oncogene, 38(7), 913-934 (2018-09-09)
The propensity of cancer cells to transition between epithelial and mesenchymal phenotypic states via the epithelial-mesenchymal transition (EMT) program can regulate metastatic processes, cancer progression, and treatment resistance. Transcriptional investigations using reversible models of EMT, revealed the mesenchymal-to-epithelial reverting transition
Molecular systems biology, 13(11), 953-953 (2017-11-08)
Cellular metabolic demands change throughout the cell cycle. Nevertheless, a characterization of how metabolic fluxes adapt to the changing demands throughout the cell cycle is lacking. Here, we developed a temporal-fluxomics approach to derive a comprehensive and quantitative view of
Journal of molecular and cellular cardiology, 72, 296-304 (2014-04-17)
Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) have great potential as a cell source for therapeutic applications such as regenerative medicine, disease modeling, drug screening, and toxicity testing. This potential is limited, however, by the immature state of
Scientific reports, 7(1), 13101-13101 (2017-10-14)
Adipose tissue takes up glucose and releases lactate, thereby contributing significantly to systemic glucose and lactate homeostasis. This implies the necessity of upregulation of net acid and lactate flux capacity during adipocyte differentiation and function. However, the regulation of lactate-
EMBO molecular medicine, 10(1), 76-90 (2017-11-29)
The neural cells and factors determining normal vascular growth are not well defined even though vision-threatening neovessel growth, a major cause of blindness in retinopathy of prematurity (ROP) (and diabetic retinopathy), is driven by delayed normal vascular growth. We here
实验方案
Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
Cultrex® 3-D Culture Matrix™ RGF BME is the first Basement Membrane Extract produced and qualified specifically for use in 3D culture studies.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门