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Merck

D5030

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员,可以根据应用灵活优化配方。
杜氏改良Eagle培养基(DMEM)是对Eagle基础培养基 (BME)的改良,含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

應用

杜氏改良Eagle′培养基已用于:
  • 培养分离的指短屈肌(FDB)纤维,以测量外源脂肪酸(FA)的利用率,作为耗氧率(OCR)测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞,制备培养人肝细胞癌HepG2细胞的低糖培养基

數量

按照每升培养基8.3克粉末配制。

重構

补充1.0g/L葡萄糖,0.584g/L L-谷氨酰胺,3.7g/L碳酸氢钠。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

Cultrex® 3-D Culture Matrix™ RGF BME is the first Basement Membrane Extract produced and qualified specifically for use in 3D culture studies.

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