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Merck

R4130

SAFC

RPMI-1640 培养基

HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

數量

按照每升培养基16.4克粉末配制。

重構

补充2.0 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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