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产品名称
RPMI-1640 培养基, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
品質等級
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
應用
RPMI-1640培养基已用于:
- 培养PC-3前列腺细胞系和白血病细胞
- 维持三阴性MDA-MB-231和管腔型MCF7白种人乳腺癌细胞系
- 培养循环肿瘤细胞(CTC)
重構
补充 0.3 g/L L-谷氨酰胺。
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产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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