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產品線
BioReagent
形狀
powder
成份
Dye content, ~85%
技術
protein staining: suitable
雜質
≤10% water
適合性
suitable for electrophoresis
SMILES 字串
[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI 密鑰
AOMZHDJXSYHPKS-DROYEMJCSA-L
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相关类别
應用
萘酚蓝黑已被用于蛋白质印迹膜的染色，可用于检测转移至膜的所有蛋白质。
生化／生理作用
萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后，建议将蛋白固定在凝胶中。然后可以将凝胶用0.1％萘酚蓝黑在7％（v/v）的乙酸中染色至少2小时，并用7％（v/v）乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20％。
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1B
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
其他客户在看
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Teresa M Lamb et al.
G3 (Bethesda, Md.), 3(12), 2273-2280 (2013-10-22)
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled
Staining of proteins on gels: comparisons of dyes and procedures.
C M Wilson
Methods in enzymology, 91, 236-247 (1983-01-01)
H Towbin et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350-4354 (1979-09-01)
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was
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