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Merck

G1041

Sigma-Aldrich

EZBlue 凝胶染色试剂

别名:

protein gel stain, protein stain

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About This Item

分類程式碼代碼:
41105300
NACRES:
NA.32

技術

protein staining: suitable

品質等級

儲存溫度

2-8°C

一般說明

EZBlue 考马斯亮蓝 G-250 胶体蛋白染色方便、灵敏、安全,在显著减少染色时间的同时,改善了蛋白电泳结果。EZBlue包装方便, 不需要忙乱的称重或添加甲醇或酸。作为胶体染色剂,它只与蛋白质反应,而不与凝胶本身反应。背景染色减少,因此蛋白条带几乎可以立即显示出来。无需脱色步骤,但水洗可能会强化条带并澄清背景。最令人印象深刻的是,EZBlue 极为灵敏,可检测低至 5 ng 的蛋白质。

應用

EZBlue 凝胶染色试剂已被用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 中的染色试剂。

特點和優勢

  • 预混溶液 省去了制备染色剂所需的时间和精力
  • 灵敏度的提高 确保低丰度蛋白(低至 5 ng)可被检测到
  • 快速反应 显著减少了染色和冲洗所需的时间
  • 无溶剂废物 ,因此可以通过消除有害物质处置步骤来节省时间和金钱

其他說明

即用型亮蓝 G-250 基蛋白染色剂,用于聚丙烯酰胺凝胶和 PVDF 膜上的“一步”超灵敏检测。

法律資訊

EZBlue is a trademark of Sigma-Aldrich Co. LLC
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardCorrosion

訊號詞

Warning

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

標靶器官

Eyes,Central nervous system

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Rakhi Panda et al.
Journal of agricultural and food chemistry, 63(43), 9629-9639 (2015-10-09)
Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this
Antibodies to the GABA(B) receptor in limbic encephalitis with seizures: case series and characterisation of the antigen.
Lancaster E, et al.
Lancet Neurology, 9, 67-76 (2010)
AMPA receptor antibodies in limbic encephalitis alter synaptic receptor location.
Lai M, et al.
Annals of Neurology, 65, 424-434 (2009)
Praveen Maurye et al.
Electrophoresis, 38(16), 2060-2068 (2017-04-27)
PAGE is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and
Nathan L Marsteller et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 141, 111398-111398 (2020-05-22)
Currently no validated animal model is predictive of human responses in ranking purified dietary proteins in the prevalence or potency of food allergy in humans. Since the gastrointestinal microbiota is thought to influence oral tolerance, we hypothesize that a germ-free

商品

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.

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