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Merck

PROTSIL2

Sigma-Aldrich

ProteoSilver Plus 银染试剂盒

别名:

protein stain, silver stain

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About This Item

分類程式碼代碼:
41105322
NACRES:
NA.32

用途

 kit sufficient for 25 mini-gels (10 × 10 cm)

技術

protein staining: suitable

儲存溫度

20-25°C

一般說明

ProteoSilver Plus银染试剂盒具有方便的包装、极稳定的性能和高灵敏度,是所有蛋白质研究科学家的理想选择。 与ProteoSilver(PROTSIL1)相似,该试剂盒含有制备好的银染试剂,并配有如何获得最佳结果的详细说明。 ProteoSilver Plus的检出限为0.1 ng/mm2 的蛋白质(BSA),本底极低,可对低丰度蛋白进行出色的检测。
ProteoSilver Plus也可用于MALDI-MS检测,其不含会使赖氨酸残基发生交联的戊二醛,该成分会导致质谱结果失准。ProteoSilver Plus银染试剂盒另外含有两种可使切离蛋白点脱色的试剂,适于希望通过胰蛋白酶消化和MALDI-MS分析来进一步研究蛋白特性的研究人员。

應用

ProteoSilver 银染试剂盒已可用于凝胶染色,以便观察显色的蛋白质。该试剂盒提供的脱色剂可用于使感兴趣的蛋白脱色,以便进行质谱分析。

特點和優勢

  • 经预混和预称重的溶液可减少对每一成分单独进行采购及制备所花费的时间和成本。
  • 经优化的实验方案 有助于确定获得最佳结果所需的条件。
  • 其高度的灵敏度及极低的本底可确保对低丰度蛋白的检测并从其他蛋白中分辨出来。
  • 兼容于MALDI感兴趣蛋白点可通过质谱进行进一步的定性分析。
  • 室温稳定性 使得其保存容易而且方便。

法律資訊

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

標靶器官

Eyes,Central nervous system, Respiratory system

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

149.0 °F - closed cup

閃點(°C)

65 °C - closed cup


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Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
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International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
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The increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour.
Peeping into human renal calcium oxalate stone matrix: characterization of novel proteins involved in the intricate mechanism of urolithiasis.
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Slovénie Pyndiah et al.
Molecular & cellular proteomics : MCP, 6(2), 193-206 (2006-11-10)
The study of protein interactions constitutes an important domain to understand the physiology and pathogenesis of microorganisms. The two-dimensional blue native/SDS-PAGE was initially reported to analyze membrane protein complexes. In this study, both cytoplasmic and membrane complexes of a bacterium

商品

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

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