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Merck
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Key Documents

B2025

Sigma-Aldrich

亮蓝 G-胶体浓缩液

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.85

形狀

solution

品質等級

200

儲存溫度

room temp

相关类别

一般說明

考马斯亮蓝染色剂在十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE) 中被广泛用于蛋白质的检测。亮蓝 G 染色因其灵敏度接近银染法而被称为蓝银染色。考马斯亮蓝 G 染色液通过疏水作用与蛋白质结合。它可以有效染色角膜前囊膜，毒性相对较小。

應用

亮蓝 G-胶体浓缩液用于在分析酿酒酵母中的
  • 转录因子（TFIID）复合物前，进行体外胃蛋白酶消化后卵巢肿瘤液蛋白质组中
    • 的蛋白质片段的染色

    • 重構

    用 800 mL 水稀释至 1 L 后，混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。

    分析報告

    已在 SDS-PAGE 上检测了本品的适用性。

    相關產品

    产品编号
    说明
    价格

    象形圖

    Corrosion
    GHS05

    訊號詞

    Warning

    危險聲明

    H290,H315,H319

    危險分類

    Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

    儲存類別代碼

    8B - Non-combustible corrosive hazardous materials

    水污染物質分類（WGK）

    WGK 2

    閃點（°F）

    Not applicable

    閃點（°C）

    Not applicable

    分析证书(COA)

    输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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    V Neuhoff et al.
    Electrophoresis, 9(6), 255-262 (1988-06-01)
    An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations
    Staining ability and biocompatibility of brilliant blue G: preclinical study of brilliant blue G as an adjunct for capsular staining
    Hisatomi T, et al.
    Archives of Ophthalmology, 124(4), 514-519 (2006)
    Antonis Ververis et al.
    Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
    Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
    Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
    Dyballa N and Metzger S
    Journal of Visualized Experiments (2009)
    A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins
    Thomas K, et al.
    Regulatory Toxicology and Pharmacology, 39(2), 87-98 (2004)
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    实验方案

    Brilliant Blue G – Colloidal Concentrate Protocol

    Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity.

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