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product name
4-硝基苯基磷酸盐 二钠盐 六水合物, tablet
形狀
tablet
品質等級
溶解度
water: 1 tablet/3 mL, clear, colorless
儲存溫度
−20°C
SMILES 字串
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI 密鑰
KAKKHKRHCKCAGH-UHFFFAOYSA-L
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相关类别
一般說明
用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。
應用
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.
生化/生理作用
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
數量
Contains 15 mg substrate per tablet
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines
Cytokine, 91, 1-5 (2017)
Molecular cell, 55(3), 397-408 (2014-07-16)
All metabolic activities operate within a narrow pH range that is controlled by the CO2-bicarbonate buffering system. We hypothesized that pH could serve as surrogate signal to monitor and respond to the physiological state. By functionally rewiring the human proton-activated
Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol
Analytical Chemistry, 90(10), 6339-6345 (2018)
Protein tyrosine phosphatase assays
Current Protocols in Immunology, 93(1), 11-17 (2011)
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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