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一般說明
本品是由 对硝基苯基磷酸酯(pNPP)组成的缓冲碱性磷酸酶底物。这个先进的基质系统含有不溶性稳定剂,可提高稳定性和反应性,并降低基线吸收。它产生一种可溶性终产物,因此不推荐用于蛋白质印迹或组织化学应用。
應用
产生一种可溶性黄色反应产物,可在405nm处读取。对于终点测定,可以使用3N NaOH终止反应。推荐用于ELISA(微孔)分析;不建议用于膜应用。
注意
可在2-8°C下稳定保存至少一年。
外觀
即用型
準備報告
含有对硝基苯基磷酸酯,它是碱性磷酸酶的底物。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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