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一般說明
MystiCq® microRNA SYBR® Green qPCR ReadyMix™是MystiCq microRNA qPCR 检测系统的成分之一。该预混液是一种专门配制的 2X 浓缩液，含有基于抗体的热启动 Taq DNA 聚合酶以及高特异性、可靠和可重复的 microRNA qPCR 测定所需的所有优化成分。只需添加特定的 MystiCq microRNA qPCR 检测引物、MystiCq Universal PCR 引物和由 MystiCq microRNA cDNA 合成试剂盒生成的 cDNA 模板，′即可开始使用。由于不同的实时 PCR 仪器有不同的荧光标准化方法，您的平台可以使用 MystiCq microRNA SYBR Green qPCR ReadyMix。
應用
MystiCq® microRNA SYBR® Green qPCR ReadyMix™ may be used in reverse transcription-quantitative polymerase chain reaction (RT-PCR) to measure miRNA expression levels in different cell lines.
特點和優勢
- MystiCq microRNA qPCR 检测试剂专用引物、对照引物和通用 PCR 引物
- 采用热启动 Taq、dNTP 和优化的缓冲液，进行 2 次浓缩，便于反应设置
- 包括最佳浓度的被动参比染料（如需要）
- 该预混液可以很容易地扩增400到500 bp的片段；但是，要得到最好的结构，扩增子大小应限制在80 - 200 p。
包裝
默认反应体积为 50μL
100RXN 包装为 2 X 1.25 mL
500RXN 包装为 10 X 1.25 mL
1 X 50 mL 包装为 1 X 50 mL
100RXN 包装为 2 X 1.25 mL
500RXN 包装为 10 X 1.25 mL
1 X 50 mL 包装为 1 X 50 mL
其他說明
MystiCq® microRNA SYBR® Green qPCR ReadyMix™, iQ™, is compatible with the following platforms: Biorad iCycler iQ, BioRad iQ5, BioRad MyiQ™.
法律資訊
MyiQ is a trademark of Bio-Rad Laboratories, Inc.
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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