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Merck

MAK505

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

應用

food and beverages
pharmaceutical

檢測方法

colorimetric
fluorometric

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damage.

特點和優勢

  • Linear detection range: 6 to 1000 µM for the colorimetric assay and 1 to 100 µM for the fluorometric assay.

適合性

The kit is suitable for ascorbic acid determination in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on ascorbic acid metabolism.

原則

The Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the ascorbic acid concentration in the sample.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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