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用途
sufficient for 2,500 colorimetric tests
檢測方法
colorimetric
相關疾病
cardiovascular diseases; gastrointestinal diseases
儲存溫度
2-8°C
一般說明
磷酸盐是人体内含量最丰富的一种矿物质,含量约占总体重的1%。甲状旁腺激素(PTH)、钙三醇和调磷因子等多种调节剂共同维持磷酸盐稳态。维持磷酸盐稳态对于各种生物过程至关重要,生物过程包括能量代谢、细胞信号传导、蛋白质合成调节、骨骼发育和骨骼完整。†生长板中成熟软骨细胞的凋亡过程需要足够水平的磷酸盐。磷酸盐缺乏与骨骼病理和临床疾病有关。†
應用
孔雀石绿磷酸盐检测试剂盒已被用于测定以下应用中游离正磷酸盐的释放:
- 磷酸酶检测:从肽、蛋白质或小分子底物中释放磷酸盐。
- 脂肪酶检测:从磷脂中释放磷酸盐
- 核苷三磷酸酶检测:从核苷三磷酸(ATP、GTP、TTP、CTP等)中释放磷酸盐。
- 磷脂、蛋白质和DNA等中磷酸盐的定量。
- 药物发现:磷酸酶抑制剂的高通量筛选。
孔雀石绿磷酸盐测定试剂盒用于磷酸盐定量 和稳态动力学研究。
特點和優勢
能与高通量处理系统兼容。
適合性
适用于各种生物和环境样品中的磷酸盐测定。
原則
孔雀石绿是一种属于三苯甲烷基团的碱性染料。它在生物学中可用于显微镜样品的染色,例如植物组织、酵母和细菌。孔雀石绿法是检测磷酸盐释放的灵敏方法。孔雀石绿磷酸盐测定试剂盒基于对孔雀石绿、钼酸盐和游离正磷酸盐之间形成的绿色复合物的定量。可以在分光光度计(600-660nm)或读板器上对反应中快速颜色形成进行方便地测定。该非放射性比色测定试剂盒已经过优化,可提供卓越的灵敏度和更长的保质期。该测定简单且快速,只需要单一的添加步骤,以对磷酸盐进行测定。可以在管、比色皿或多孔板中进行测定。可以在96孔和384孔板中方便地进行测定,以用于酶抑制剂的高通量筛选。
訊號詞
Warning
危險分類
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
3 - Flammable liquids
閃點(°F)
82.4 °F
閃點(°C)
28 °C
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