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Merck
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主要文件

AA0100

Sigma-Aldrich

氨检测试剂盒

sufficient for 100 assays

别名:

氨定量检测试剂盒

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.84

用途

sufficient for 100 assays

儲存溫度

2-8°C

應用

在一项评估铬代谢性酸中毒的响应过程中肾氨转运体表达情况的研究中，用户使用了Sigma氨检测试剂盒来测定血清和尿液中的氨浓度。
本试剂盒可用于酶促定量分析、测定食品及其他生物材料中的氨水平。 L-谷氨酸脱氢酶(GIDH)存在的情况下，氨能够与α-酮戊二酸(KGA)和还原型烟酰胺腺嘌呤二核苷酸磷酸盐(NADPH)反应，生成L-谷氨酸和氧化型烟酰胺腺嘌呤二核苷酸磷酸盐(NADP+)。340 nm波长处吸光值下降是NADPH的氧化所致，具体的吸光度与样品中的氨浓度成正比。 L-谷氨酸脱氢酶能够与氨特异性地反应。氨检测试剂盒可用于测定0.2-15 μg/ml的氨浓度。

仅试剂盒组分

产品编号
说明
  • Ammonia Assay Reagent 10 vial(s)
  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
  • Ammonia Standard Solution 1 mL/vial

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H319,H334

危險分類

Eye Irrit. 2 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH3833
SLCH1703
SLCG9432
SLCG9434
SLCF6398

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Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.
Seshadri, R.
American Journal of Physiology: Renal Physiology, 290, F397-F408 (2006)
Bergmeyer, H.U., and Beutler, H-O.
Methods of Enzymatic Analysis, 454-461 (1985)
Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
W E Neeley et al.
Clinical chemistry, 34(9), 1868-1869 (1988-09-01)
This is an automated, cost-efficient enzymatic method for determining ammonia in plasma. In most assays for ammonia the reagents are stable for only one or two days at 4 degrees C, but the reagents in our method are stable for
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