推荐产品
用途
sufficient for 100 tests (bioluminescent)
應用
pharmaceutical
檢測方法
chemiluminescent
相關疾病
cancer
儲存溫度
−20°C
一般說明
ADP/ATP比例变化已用于区分细胞死亡和活率模式。ATP水平升高、ADP水平降低表示细胞正在增殖。相反，ATP降低、ADP的升高表示细胞凋亡或坏死，且相对于凋亡，坏死细胞ATP降低和ADP升高程度更为明显。
應用
- LOC554202 contributes to chordoma progression by sponging miR-377-3p and up-regulating SMAD3.: This article investigates the molecular mechanisms by which LOC554202 facilitates chordoma progression through miR-377-3p and SMAD3 regulation. The ADP/ATP Ratio Assay Kit was utilized to assess the metabolic impact of these molecular changes on cell viability and proliferation (Xu et al., 2023).
- FOXG1 improves mitochondrial function and promotes the progression of nasopharyngeal carcinoma.: This study explores how FOXG1 enhances mitochondrial function, thereby promoting nasopharyngeal carcinoma progression. The ADP/ATP Ratio Assay Kit was employed to measure mitochondrial function and energy metabolism changes, providing insights into the bioenergetic profile of cancer cells (Xi et al., 2021).
特點和優勢
兼容高通量的操作系统。
適合性
适于检测细胞中的凋亡和坏死情况，或研究化合物对细胞增殖的影响。
原則
ADP/ATP比率测定试剂盒提供了一种简单直接的细胞ADP和ATP水平测定方法，可用于筛查细胞凋亡、坏死和增殖。该测定包括两个步骤。第一步，利用工作试剂裂解细胞以释放ATP和ADP。在存在萤光素酶的条件下，ATP立即与底物D-萤光素反应产生荧光。光强度是细胞内ATP浓度的直接指标。
萤光素酶
ATP + D-萤光素 + O2 ----------> 氧化萤光素 + AMP + PPi + CO2 + 荧光
第二步，ADP通过酶解反应转化为ATP。然后像第一步一样，新形成的ATP与D-荧光素反应。测得的第二个光强度代表样品中的总ADP和ATP浓度。
萤光素酶
ATP + D-萤光素 + O2 ----------> 氧化萤光素 + AMP + PPi + CO2 + 荧光
第二步，ADP通过酶解反应转化为ATP。然后像第一步一样，新形成的ATP与D-荧光素反应。测得的第二个光强度代表样品中的总ADP和ATP浓度。
相關產品
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
King-Chuen Wu et al.
Journal of cellular biochemistry, 118(5), 1108-1117 (2016-09-09)
Cells switch to anaerobic glycolysis when there is a lack of oxygen during brain ischemia. Extracellular pH thus drops and such acidosis causes neuronal cell death. The fate of astrocytes, mechanical, and functional partners of neurons, in acidosis is less
Feilong Wang et al.
EBioMedicine, 30, 303-316 (2018-02-22)
Classical activation of M1 macrophages with lipopolysaccharide (LPS) is associated with a metabolic switch from oxidative phosphorylation to glycolysis. However, the generalizability of such metabolic remodeling to other modes of M1 macrophage stimulation, e.g. type II interferons (IFNs) such as
Aline G Cozer et al.
Lipids, 51(11), 1303-1307 (2016-10-25)
The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of
Nidal Zeineh et al.
Cells, 8(7) (2019-07-13)
The 18 kDa translocator protein (TSPO) is an initiator of the mitochondrial apoptosis cascade. Cigarette smoke (CS) exposure provokes alterations in TSPO expression as well as upregulation of its related functions such as mitochondrial membrane potential (ΔψM) and reactive oxygen
Sangiliyandi Gurunathan et al.
Polymers, 11(4) (2019-04-26)
Metal nanoparticles and the combination of metal nanoparticles with graphene oxide are widely used in environmental, agriculture, textile, and therapeutic applications. The effect of graphene oxide-green platinum nanoparticles (GO-PtNPs) on human prostate cancer cells (LNCaP) is unclear. Therefore, this study
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门