推荐产品
用途
sufficient for 200 fluorometric tests (Blue fluorescence)
應用
cosmetics
food and beverages
檢測方法
fluorometric
儲存溫度
−20°C
相关类别
一般說明
焦磷酸 (PPi) 通过多次生化反应而生出，如 ATP 水解、DNA 和 RNA 聚合、环 AMP 形成以及脂肪酸-辅酶 a 酯的形成。其被无机焦磷酸酶水解。PPi 存在于血液和组织的细胞外基质中。
適合性
焦磷酸检测试剂盒为用户提供了简单直接的微孔板分析方法，可测定多种样本中的焦磷酸水平。
原則
通过使用独特的荧光焦磷酸传感器测定样品的焦磷酸浓度，其中焦磷酸的存在导致产生与焦磷酸成比例的荧光产物 (λex = 316/λem = 456 nm)。该检测方法比传统的基于酶的方法更简单、更耐用，是筛选酶活性或酶抑制剂的理想方法。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
188.6 °F
閃點（°C）
87 °C
其他客户在看
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Tyler M Weaver et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25494-25504 (2020-10-02)
During DNA replication, replicative DNA polymerases may encounter DNA lesions, which can stall replication forks. One way to prevent replication fork stalling is through the recruitment of specialized translesion synthesis (TLS) polymerases that have evolved to incorporate nucleotides opposite DNA
Young-Joo Yi et al.
PloS one, 7(4), e34524-e34524 (2012-04-10)
Inorganic pyrophosphate (PPi) is generated by ATP hydrolysis in the cells and also present in extracellular matrix, cartilage and bodily fluids. Fueling an alternative pathway for energy production in cells, PPi is hydrolyzed by inorganic pyrophosphatase (PPA1) in a highly
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门