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Merck

MAK014

Sigma-Aldrich

葡萄糖-6-磷酸测定试剂盒

sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

相關疾病

hematological disorder

儲存溫度

−20°C

相关类别

一般說明

葡萄糖6-磷酸 (G6P) 是进入代谢途径或储存的关键代谢中间体。葡萄糖被己糖激酶或葡糖激酶磷酸化时,或在糖原分解过程中被磷酸葡糖变位酶转化为葡萄糖-1-磷酸时,产生 G6P。G6P 位于糖酵解和戊糖磷酸途径的起点。当血糖水平高时,它也可以储存为糖原。

應用

葡萄糖-6-磷酸检测试剂盒已被用于:

  • 葡萄糖-6-磷酸(G6P)检测
  • 葡萄糖-6-磷酸(G6P)的定量测定以及果糖-6-磷酸(F6P)
  • 代谢产物分析

適合性

适用于定量各种样品中的葡萄糖-6-磷酸。

原則

G6P 被葡萄糖-6-磷酸脱氢酶利用,以 NADPH 的形式产生还原当量。这对红细胞特别重要,因为 G6PDH 缺乏可导致溶血性贫血。通过酶测定法测定 G6P,其产生与存在的 G6P 成比例的比色 (450 nm) 产物。 典型的检测灵敏度在 1-30 纳摩尔之间。

取代透過

象形圖

Health hazardCorrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials


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Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice.
Virtu C N, et al.
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商品

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

实验方案

Assay protocol for the colorimetric detection of glucose-6-phosphate in biological samples using the glucose-6-phosphate assay kit.

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