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Merck

M8537

Sigma-Aldrich

MCDB 131培养基

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

别名:

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

MCDB 131培养基, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

形狀

powder

濃度

11.6 g/L

技術

cell culture | mammalian: suitable

成分

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

MCDB-131培养基提供了一个清晰和平衡的营养环境,可选择性地促进特定细胞类型的生长。MCDB-131在补充0.7% FBSP、10 ng/ml表皮生长因子、1 μg/ml氢化可的松后,可支持人微血管内皮细胞(包括人脐静脉内皮细胞)的克隆性生长。MCDB培养基用于特定类型细胞的低蛋白或无血清生长,使用的是激素、生长因子、微量元素或低水平透析胎牛血清蛋白(FBSP)。 

應用

MCDB-131培养基已用于:

  • 作为牛视网膜内皮细胞基础培养基的组分
  • 培养牛肺微血管内皮细胞
  • 分离猪颅内内皮细胞和猪主动脉内皮细胞

數量

按照每升培养基11.6克粉末配制。

重構

补充1.18 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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