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Merck

M2154

Sigma-Aldrich

培养基 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

培养基 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

pH值

7.0-7.6 (25 °C, 209 g/L)

成分

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

應用

培养基199已用于:
  • 培养一岁Speckle Park母牛来源的瘤胃上皮细胞
  • 作为成熟培养基组分,培养牛卵巢来源的卵丘卵母细胞复合体(COC)
  • 作为杜氏改良Eagle培养基(DMEM)组分,维持新生大鼠心肌细胞(NCM)生长

重構

补充 0.1 g/L L-谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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