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Merck
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主要文件

M3769

Sigma-Aldrich

培养基 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

别名:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

應用

培养基199已用于:
  • 培养原代人脐静脉内皮细胞(HUVEC),以用于细胞活力检测
  • 冲洗小母猪子宫角,从子宫恢复第12天妊娠
  • 培养腹膜细胞和永生化人间皮细胞(MeT-5A和ATCC-CRL-9444)

數量

配制成每升培养基中含 9.4 克粉末。

重構

补充0.1克/升左旋谷氨酰胺、2.2克/升碳酸氢钠。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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