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product name
Eagle最低必需培养基, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
成分
Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
Harry Eagle 开发的最小必需培养基 (MEM) 是所有合成细胞培养基中应用最广泛的一种。这是所提供 MEM 制剂的最丰富变体。它含有全部 21 种正常氨基酸，有些浓度增加。此外，它还含有 5 种额外的维生素。当补充胎牛血清 (FBS) 时，通常用于细胞遗传学分析。
應用
最小必需培养基 Eagle 已被用于间充质干细胞、脂肪干细胞 和成骨细胞的分离。
最小必需培养基 Eagle 用于原代成骨细胞培养 和从脐血中分离多形核白细胞 (PMN)。
生化／生理作用
MEM 通常在 15 psi 下用 0.5% 细菌蛋白胨高压灭菌 20 min，冷却，然后用 NaHCO3 中和，始终支持细胞生长良好。当 MEM 和细菌蛋白胨分别进行高压灭菌时，获得了相似的结果。细胞最初以单层生长，随后成为固定悬浮液。
重構
补充 0.292 g/L L -谷氨酰胺
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产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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BMC research notes, 4, 282-282 (2011-08-13)
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The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
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