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Merck
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文件

51412C

SAFC

最低必需培养基

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

Eagle最低必需培养基, MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

描述

for research or for further manufacturing use

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable
cell culture | stem cell: suitable

成分

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

儲存溫度

2-8°C

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相关类别

一般說明

Harry Eagle研究了小鼠L细胞和HeLa细胞在培养中的基本营养需求，因此开发了含有Earle平衡盐的最低必需培养基（MEM）。他证明这些细胞需要13种氨基酸和7种维生素才能在体外生长和繁殖。MEM是Eagle早期培养基基础培养基Eagle（BME）的改良，含有更高浓度的必需营养素。这些培养基已证明能够在培养中支持多种正常细胞和转化细胞，并含有Earle平衡盐，这使其适合在含有CO2的大气中使用。

應用

最小必需培养基 (MEM) 已被用作骨髓间充质干细胞 (BMSCs) 和神经元的生长培养基。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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