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product name
Eagle最低必需培养基, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
形狀
powder
技術
cell culture | mammalian: suitable
成分
glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质浓度。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。去除钙成分后，可用于悬浮细胞培养。它是经典MEM配方的改良版。厄尔′盐类已被汉克′盐类所取代。由于碳酸氢盐浓度显著降低，因此该介质具有显著降低的缓冲能力。
應用
Eagle最低必需培养基已用于：
- 作为Hank′s MEM(HMEM)-PIPES缓冲液成分，用于小鼠中过敏毒素(anaphylatoxin)诱导耳肿的引发
- 作为小鼠海马体切片培养基(SCM)组分
- 培养豚鼠Müller细胞
數量
按照每升培养基10.6克粉末配制。
重構
补充0.35 g/L碳酸氢钠。
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产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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