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Merck

M0643

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

别名:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

它含有19种氨基酸。必需氨基酸和非必需氨基酸;L-ala、L-asn、L-asp、L-glu、L-gly、L-pro和L-ser。可能跟适合于在血清浓度降低的情况下生长贴壁细胞,或作为开发无血清培养基的基础。
最小必需培养基Eagle包含糖、无机盐、水溶性维生素和必需氨基酸。它广泛用于许多细胞培养基研究的培养基配方。它是Eagle基础培养基(BME)的改良版本,大部分氨基酸的浓度是BME的两倍。

應用

最小必需培养基Eagle已用于培养成骨样细胞系MG-63、分散心室细胞和子宫内膜腺癌Ishikawa细胞。

數量

按照每升培养基9.6克粉末配制。

重構

补充2.2 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Erika Nahomy Marino-Marmolejo et al.
BMC microbiology, 16(1), 290-290 (2016-12-14)
Pharmaceutical industry demands innovation for developing new molecules to improve effectiveness and safety of therapeutic medicines. Preclinical assays are the first tests performed to evaluate new therapeutic molecules using animal models. Currently, there are several models for evaluation of treatments
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Hongjie Fan et al.
Cells, 10(11) (2021-11-28)
Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV

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