推荐产品
種類
Type XIII
品質等級
形狀
lyophilized powder
比活性
≥15 units/mg solid
分子量
~134 kDa
成份
Protein, 40-65% biuret
儲存溫度
2-8°C
一般說明
脂肪酶是将三酰基甘油酯分解成游离脂肪酸和甘油的水解酶。
應用
当与 L-α-甘油磷酸氧化酶(G3O-301、G3O-311、G3O-321)和甘油激酶(GYK-301、GYK-311)偶联时,该酶可用于酶促测定血清中的甘油三酯。通常,在 37oC 下,反应可在 5 分钟内完成,每次试验使用 2.5 oC~3.0 单位的酶(3.0ml),pH 约为 7.0。来源于假单胞菌属的脂肪酶已被用于评估微波辐射辅助棕榈油酶促合成生物柴油的研究。
生化/生理作用
可水解甘油三酯、甘油二酯、甘油单酯(速率依次递减)。
来源于假单胞菌的脂肪酶显示出抑制人外周血中单核细胞的趋化性。
物理性質
等电点:5.95-/+0.05
抑制剂:Hg++、 Ag+、离子型去垢剂
最佳 pH 值:7.0-9.0
最佳温度:45-50oC
pH 稳定性:pH 7.0 - 9.0 (25oC, 20 小时)
热稳定性:低于 55oC(pH 7.0,10 分钟)
抑制剂:Hg++、 Ag+、离子型去垢剂
最佳 pH 值:7.0-9.0
最佳温度:45-50oC
pH 稳定性:pH 7.0 - 9.0 (25oC, 20 小时)
热稳定性:低于 55oC(pH 7.0,10 分钟)
單位定義
在 pH7.0、37℃、牛血清白蛋白存在下,一单位酶每分钟从甘油三酯中产生 1.0 μmole 甘油。
外觀
冻干品,含有 Mg+2、胆酸钠和牛血清白蛋白(作为稳定剂)
分析報告
缩二脲法测定蛋白
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Canadian journal of microbiology, 47(10), 908-915 (2001-11-23)
Eight closely related thermophilic strains were isolated from an aerobic and thermophilic treatment of swine wastes. The pleomorphic cells (short and long rods; cocci) showed peritrichous flagella, terminally swollen sporangium, and liberated spores exhibiting hairy appendages. The Gram reaction was
Bioprocess and biosystems engineering, 36(4), 443-451 (2012-08-21)
Optimal conditions for enzymatic synthesis of biodiesel from palm oil and ethanol were determined with lipase from Pseudomonas fluorescens immobilized on epoxy polysiloxane-polyvinyl alcohol hybrid composite under a microwave heating system. The main goal was to reduce the reaction time
Chemistry and physics of lipids, 146(2), 85-93 (2007-02-06)
1,2-Ethylene-di-N-n-propylcarbamate (1) is characterized as an essential activator of Pseudomonas species lipase while 1,2-ethylene-di-N-n-butyl-, t-butyl-, n-heptyl-, and n-octyl-carbamates (2-5) are characterized as the pseudo substrate inhibitors of the enzyme in the presence of the detergent taurocholate or triton X-100. The
Microbial pathogenesis, 10(3), 173-182 (1991-03-01)
Lipase was isolated from P. aeruginosa by ultrafiltration of sterile-filtered culture supernatant. Gel filtration on Sepharose 4B yielded a broad peak corresponding to a molecular mass range of 100 to 1000 kDa. Electron microscopy of a negatively stained lipase preparation
Arteriosclerosis, thrombosis, and vascular biology, 20(11), 2428-2433 (2000-11-14)
Prebeta1-high density lipoprotein (prebeta1-HDL), the initial acceptor of cell-derived cholesterol, can be generated from HDL(2) by hepatic lipase. Because bezafibrate elevates lipase activity, it may increase prebeta1-HDL at the expense of HDL(2). To answer this question, we determined the apolipoprotein
实验方案
Assay Procedure for Lipase
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门