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Merck
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主要文件

L6136

Sigma-Aldrich

脂多糖 来源于粘质沙雷氏菌

purified by phenol extraction

别名:

LPS

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About This Item

MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Serratia marcescens

品質等級

形狀

lyophilized powder

純化經由

phenol extraction

雜質

<3% Protein (Lowry)

顏色

white to yellow cast

溶解度

water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

本品为从粘质沙雷氏菌中提取的苯酚。源菌株是ATCC 21639。

生化/生理作用

脂多糖(LPS)位于膜的外层,并且暴露在无包膜菌株的细胞表面。它们有助于维持外层膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的破坏。

準備報告

本品溶于水(5 mg/ml)或细胞培养基(1 mg/ml),得到浑浊的淡黄色溶液。以盐水为溶剂时,经过涡旋并加热至70-80 oC后,得到浓度更高但仍然浑浊的溶液(20 mg/ml)。脂多糖分子可在各种溶剂中形成胶束。在水和磷酸盐缓冲盐水中,可观察到混浊的溶液。使用有机溶剂不会得到更澄清的溶液。以甲醇为溶剂将得到含漂浮物的混浊悬浮液,而以水为溶剂将得到均匀的混浊溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

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H Heremans et al.
American journal of respiratory and critical care medicine, 161(1), 110-117 (2000-01-05)
Mice given lipopolysaccharide (LPS) intravenously developed lung edema, which was maximum after 6 h. Tumor necrosis factor, interleukin 12 (IL-12), IL-6, and interferon-gamma (IFN-gamma) appeared in the serum, and levels of nitrogen oxide (NO) derivatives were increased in serum and
Mollie R Sorrell et al.
Journal of insect physiology, 126, 104097-104097 (2020-08-14)
Fragile X syndrome (FXS), caused by a mutation in the Fragile X Mental Retardation 1 (FMR1) gene, is a common form of inherited mental retardation. Mutation of the gene leads to a loss of the gene product Fragile X Mental Retardation Protein (FMRP). While a loss
M G Zenobi et al.
Journal of dairy science, 103(3), 2200-2216 (2020-01-20)
Objectives were to evaluate the effect of prepartum energy intake and peripartal supplementation of ruminally protected choline (RPC) on select indicators of immune status in blood plasma and on lipopolysaccharide-stimulated blood cells ex vivo. At 47 ± 6 d before
Ching-Hua Hsieh et al.
Journal of biomedical science, 19, 69-69 (2012-08-02)
Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole
Victor Meseguer et al.
Nature communications, 5, 3125-3125 (2014-01-22)
Gram-negative bacterial infections are accompanied by inflammation and somatic or visceral pain. These symptoms are generally attributed to sensitization of nociceptors by inflammatory mediators released by immune cells. Nociceptor sensitization during inflammation occurs through activation of the Toll-like receptor 4

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