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Merck

I7633

Sigma-Aldrich

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

别名:

IMDM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

Iscove 改良杜氏培养基, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

形狀

powder

技術

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

成分

phenol red: yes
glucose: yes
L-glutamine: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Iscove改良杜氏培养基是杜氏改良Eagle培养基(DME)的改良版本,含有硒、额外的氨基酸和维生素、丙酮酸钠、4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液、以及硝酸钾(代替硝酸铁)。据报道,Iscove培养基可支持鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和多种杂交细胞的生长。

應用

Iscove改良杜氏培养基已用于KG1a细胞(人白血病细胞株)和中国仓鼠卵细胞(CHO)DG44细胞株的培养。
据报道,其可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞的生长。

數量

配制成每升培养基中含 17.7 克粉末。

重構

补充 3.024 g/L 碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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