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一般說明
透明质酸酶有助于哺乳动物卵子受精,因此在睾丸中的含量很高。哺乳动物透明质酸酶具有催化表皮生长因子(EGF)样结构域和富含C端半胱氨酸的区域。
應用
透明质酸酶已用于:
- 用作从生精小管中分离睾丸和生殖细胞的酶混合物组分
- 预处理脱蜡腺瘤切片,以进行免疫组织化学分析
- 酶解脐带血管,从而产生脐带间质干细胞系
生化/生理作用
商业领域采用绵羊睾丸来源透明质酸酶分别解聚结缔组织和软骨中的透明质酸和硫酸软骨素。
这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-(1→4) 糖苷键。
單位定義
在 37 ºC、pH 5.35 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm),与 BSA 复合 45 分钟后测量)。
其他說明
查看更多用于复合糖分析的酶的信息,请访问 www.sigma-aldrich.com/enzymeexplorer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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