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Key Documents

GE28-9909-44

Sigma-Aldrich

Superdex® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

别名:

Superdex Increase

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

glass column
polyethylene filter

產品線

Superdex®

分子量

10000-600000 Da (globular proteins)

包裝

pkg of 1 ea

製造商／商標名

Cytiva 28-9909-44

參數

<1.8 mL/min flow rate
<1.80 mL/min flow rate
25-500 μL sample volume
3.0 Mpa (580 psi)

床體積

~24 mL

柱長度 × 內徑

30 cm × 10 mm

基質

agarose-dextran composite
polymeric

粒徑

8.6 μm

cleaning

1-14

工作範圍

3-12

分離技術

size exclusion (SEC)

一般說明

Superdex increase 200 是一种用于小规模 (mg) 制备纯化的高分辨率凝胶过滤色谱柱，也可用于表征和分析分子量在 10,000 和 600,000 之间的蛋白质，例如抗体的聚合分析、膜蛋白的粒度均一性分析、 或蛋白质-蛋白质相互作用。预装小的刚性琼脂糖微珠，可实现高流速和 pH 稳定性

適合性

可用于制备和分析目的
用于小规模 (25-500 mL) 制备纯化的凝胶过滤。
长期使用：所有常用水性缓冲液、最高达 8 M 的尿素、离子和非离子洗涤剂、最高达 6M 的盐酸胍

儲存和穩定性

4至30 °C下储存。（20%乙醇）
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

推薦產品

探索最适于 HPLC 或 LC-MS 分析的LiChropur试剂

法律資訊

Superdex is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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商品

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

实验方案

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

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