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材料
bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)
分子量
10000-600000 Da (globular proteins)
包裝
pkg of 1 ea
製造商/商標名
Cytiva 28-9893-36
參數
<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa
床體積
320 mL
柱長度 × 內徑
60 cm × 26 mm
基質
agarose-dextran composite
dextran-agarose particle platform
polymeric
基質活性組
dextran-agarose phase
dextran-agarose phase
樣品平均尺寸
34 μm
cleaning
1-14
工作範圍
3-12
分離技術
size exclusion (SEC)
一般說明
Superdex ®制备级(pg)是由葡聚糖与高度交联的琼脂糖共价键合而成。这些介质的分离特性由葡聚糖成分决定。陡峭的选择性曲线为以下分子量范围内的肽和蛋白质提供出色的分辨能力:Mr <10 000 (Superdex®30 pg)、Mr 3 000 至 70 000 (Superdex® 75 pg) 和 Mr 10 000 600 000 (Superdex ®200 pg)。该介质结合了高机械强度和高亲水性,可实现高流速和最小的非特异性相互作用。
特點和優勢
- 分辨率高,运行时间短,回收率好。
- 高流速下的高容量。
- 分离可以扩大到生产规模。
- 预填充柱实现方便性和重现性。
- 1/16” 接头方便连接到 AKTA 设计系统。
適合性
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
实验方案
Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
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