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Key Documents

GE28-9356-05

Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-400 HR

Cytiva 28-9356-05, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

polypropylene

分子量

20000-8000000 Da (globular proteins)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9356-05

參數

<1.3 mL/min flow rate
<13 mL sample volume
0.15 Mpa (22 psi) (Over the Packed Bed During Operation)

床體積

320 mL

柱長度 × 內徑

60 cm × 26 mm

基質

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基質活性組

dextran-acrylamide phase

樣品平均尺寸

50 μm

cleaning

2-13

工作範圍

3-11

分離技術

size exclusion (SEC)

一般說明

HiPrep 26/60 Sephacryl S-400 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 ml sample volume. Sephacryl S-400 is optimized for separating polysaccarides, macromolecules with extended structures, and even small plasmids

適合性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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实验方案

This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.

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