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Merck

GE17-1166-01

Sigma-Aldrich

HiPrep 16/60 Sephacryl® S-200 HR

Cytiva 17-1166-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

别名:

Sephacryl S-200 column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

polypropylene

分子量

5000-250000 Da (globular proteins)

包裝

pkg of 1 ea

製造商/商標名

Cytiva 17-1166-01

參數

<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)
22 psi

床體積

120 mL

柱長度 × 內徑

60 cm × 16 mm

基質

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基質活性組

dextran-acrylamide phase

樣品平均尺寸

50 μm

cleaning

2-13

工作範圍

3-11

分離技術

size exclusion (SEC)

一般說明

HiPrep 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-200 is used for separating midsize proteins including antibodies and serum proteins.

特點和優勢

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

適合性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析证书(COA)

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实验方案

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.

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