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Key Documents

GE29-0915-98

Sigma-Aldrich

Superose® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

别名:

Size exclusion chromatography, Superose 6 column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

glass column

產品線

Superose®

分子量

5000-5000000 Da (globular proteins)

製造商／商標名

Cytiva 29-0915-98

參數

<0.03 mL/min flow rate (20% ethanol at low temp)
<0.07 mL/min flow rate (20% ethanol at room temp)
<0.07 mL/min flow rate (water at low temp)
<0.15 mL/min flow rate (water at room temp)
3 Mpa (435 psi)
4-50 μL sample volume

基質

cross-linked agarose
polymeric

基質活性組

agarose phase

樣品平均尺寸

8.6 μm

cleaning

1-14

工作範圍

3-12

分離技術

size exclusion (SEC)

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相关类别

一般說明

Superose® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

特點和優勢

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

適合性

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

儲存和穩定性

Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

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商品

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

实验方案

Performing a Separation with Superose

Superose from GE Healthcare are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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