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Merck

S500HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

500-HR, MW range 40-20,000 kDa (dextrans)

别名:

聚([丙烯基葡聚糖]-co- N,N′-亚甲基双丙烯酰胺)

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

product name

Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)

描述

stability: stable to 0.2M NaOH

形狀

suspension (20% aqueous ethanol)

特點

autoclavable

基質

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

粒徑

25-75 μm (wet)

孔徑

40-20,000 kDa MW range (dextrans)

相容性

mode of use size exclusion chromatography

儲存溫度

2-8°C

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一般說明

  • 肿胀,悬浮在 20% 乙醇水溶液中
  • 高压灭菌
  • 在 0.2 N 氢氧化钠 (NaOH) 中稳定
  • 冷藏温度 (2-8°C) 下储存
S500HR-750ML′s updated product number is GE17-0613-01

應用

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
用于分子排阻色谱;HR级是较小的粒径,具有较窄的大小范围分布,能够以更快的流速实现更有效的分离。 已列出了球状蛋白质和葡聚糖的推荐分馏范围。

法律資訊

Sephacryl is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

100.4 - 109.4 °F - closed cup

閃點(°C)

38 - 43 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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M A Karymov et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(14), 2631-2641 (2001-12-01)
Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin
Theresa L Gioannini et al.
The Journal of biological chemistry, 277(49), 47818-47825 (2002-10-10)
Experiments utilizing endotoxin aggregates, lipooligosaccharides (LOS) isolated from metabolically labeled Neisseria meningitidis serotype group B, demonstrate that albumin is an essential component of lipopolysaccharide binding protein- (LBP) and sCD14-dependent 1) disaggregation of LOS and 2) LOS activation of human umbilical
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)
Xiaolei Wang et al.
Molecular microbiology, 83(1), 195-207 (2011-11-25)
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.

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