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Key Documents

S200HR

Sigma-Aldrich

Sephacryl®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

别名:

聚([丙烯基葡聚糖]-co- N,N′-亚甲基双丙烯酰胺)

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.56

描述

stability: stable to 0.2M NaOH

形狀

suspension (20% aqueous ethanol)

特點

autoclavable

基質

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

粒徑

25-75 μm (wet)

孔徑

1-80 kDa MW range (dextrans)
5-250 kDa MW range (globular proteins)

相容性

mode of use size exclusion chromatography

儲存溫度

2-8°C

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一般說明

  • 肿胀,悬浮在 20% 乙醇水溶液中
  • 高压灭菌
  • 在 0.2 N 氢氧化钠 (NaOH) 中稳定
  • 冷藏温度 (2-8°C) 下储存
S200HR-750 mL′s 更新后的产品编号为 GE17-0584-01

應用

Sephacryl ® 用于凝胶过滤色谱、蛋白质色谱以及树脂和分离介质。Sephacryl ® 已被用于开发一种用于镰状细胞病药物发现的新型高通量筛选分析。Sephacryl ® 也被用于纯化和表征绵羊胰腺弹性蛋白酶以及人尿液中的溶菌酶。
用于分子排阻色谱;HR级是较小的粒径,具有较窄的大小范围分布,能够以更快的流速实现更有效的分离。 已列出了球状蛋白质和葡聚糖的推荐分馏范围。

法律資訊

Sephacryl is a registered trademark of Cytiva

相關產品

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

100.4 - 109.4 °F - closed cup

閃點(°C)

38 - 43 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)-binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine monophosphate (GMP) phosphodiesterase activity. We have cloned, overexpressed and purified this protein, which we
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The Journal of experimental biology, 207(Pt 26), 4633-4649 (2004-12-08)
The eye lenses of the Antarctic nototheniid fishes that inhabit the perennially freezing Antarctic seawater are transparent at -2 degrees C, whereas the cold-sensitive mammalian and tropical fish lenses display cold-induced cataract at 20 degrees C and 7 degrees C
D J Flavell et al.
British journal of cancer, 84(4), 571-578 (2001-02-24)
Severe combined immunodeficient (SCID) mice injected i.v. with the human T-ALL cell line CCRF CEM (SCID-CEM mice) develop within 50 days life-threatening multi-organ growth of leukaemia cells. The development of leukaemia in SCID-CEM mice treated with three 10 microg i.v.
M Hamilton et al.
The Journal of biological chemistry, 276(31), 29079-29090 (2001-05-19)
Phorbol ester stimulation of the MAPK cascade is believed to be mediated through the protein kinase C (PKC)-dependent activation of Raf-1. Although several studies suggest that phorbol ester stimulation of MAPK is insensitive to dominant-negative Ras, a requirement for Ras

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