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Key Documents

GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

别名:

affinity chromatography resin, GST-tagged proteins purification

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

glutathione

包裝

pack of 100 mL

製造商/商標名

Cytiva 17-0756-05

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

4-13

工作範圍

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

特點和優勢

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

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实验方案

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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