GSTrap^™ 4B

Name: GSTrap<SUP>™</SUP> 4B
Brand: SIGMA

Cytiva 29-0486-09, pack of 1 mL

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About This Item

分類程式碼代碼:

23151817

NACRES:

NA.56

ligand

glutathione

包裝

pack of 1 mL

製造商／商標名

Cytiva 29-0486-09

存貨情形

not available in North America

參數

42 psi (H₂O at 25°C)

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

4-13

工作範圍

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C)

一般說明

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

應用

Tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System.

特點和優勢

High binding capacity.
Mild elution conditions preserving protein antigenicity and function.
Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.