推荐产品
ligand
glutathione
包裝
pack of 25 mL
製造商/商標名
Cytiva 17-5279-01
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
3-12
工作範圍
3-12
容量
>7 mg/mL binding capacity(medium)
一般說明
Glutathione Sepharose™ High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.
特點和優勢
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
- Mild conditions during the purification process preserves protein antigenicity and function
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
实验方案
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
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