推荐产品
ligand
glutathione
包裝
pack of 5 × 1 mL
製造商/商標名
Cytiva 17-5281-01
參數
42 psi (H2O at room temperature.)
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
3-12
工作範圍
3-12
容量
>7 mg/mL binding capacity(H2O at room temperature.)
一般說明
應用
GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.
特點和優勢
- High resolution purification of GST-tagged Proteins.
- Mild elution conditions preserving Protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
儲存和穩定性
分析報告
其他說明
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
实验方案
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
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