推荐产品
ligand
glutathione and 10-carbon linker arm
包裝
pack of 100 mL
製造商/商標名
Cytiva 17-5132-02
基質
highly cross-linked 4% agarose
平均直徑
90 μm
cleaning
3-12
工作範圍
3-12
容量
>10 mg/mL binding capacity
一般說明
Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.
特點和優勢
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
实验方案
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
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