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一般說明
葡萄糖-6-磷酸脱氢酶(G-6-PDH)由His-Asp催化二联体构成,并以同二聚体形式存在。结构上,G-6-PDH在辅酶结合结构域内包含罗斯曼折叠构成的二核苷酸结合区域。还具有一个较大的β + α结构域,在374位有一个独特的天冬氨酸残基。
應用
来自肠膜明串珠菌的葡萄糖-6-磷酸脱氢酶已联用己糖激酶进行小鼠肝样品中的葡萄糖测定。
生化/生理作用
葡萄糖-6-磷酸脱氢酶(G-6-PDH)可使用烟酰胺腺嘌呤二核苷酸磷酸(NADP+)或NAD+作为辅酶,在细菌代谢中具有重要作用。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯,作为磷酸戊糖途径的第一步。
單位定義
一个单元在NAD 存在下,在pH 7.8,30℃ 下,每分钟将 1.0 μmole的D-葡萄糖-6-磷酸氧化成 6-磷酸-D-葡萄糖酸盐。
外觀
含有Ficoll和Tris 缓冲盐的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
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实验方案
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
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