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一般說明
β-半乳糖苷酶是一个四聚体,由四个相同的亚基组成,每个亚基的分子量都为135,000。它是一种含巯基的酶,每个亚基含有19个半胱氨酸残基。
應用
β-半乳糖苷酶可与特异性识别靶分子的抗体偶联(酶免疫测定或EISA)。β-半乳糖苷酶也可用作报告酶以监测启动子的基因表达水平。它可以用作具有抗β-半乳糖苷酶抗体的阳性对照蛋白。
生化/生理作用
β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。
物理性質
四聚体的分子量为465 kDa(每个亚基116.3 kDa)
單位定義
在37 °C,pH 7.3条件下,一个单位每分钟可将1.0μmole邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和D-半乳糖。
外觀
含有Tris缓冲盐和氯化镁
基底
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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