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Merck

G5635

Sigma-Aldrich

β 半乳糖苷酶 来源于大肠杆菌

Grade VIII, lyophilized powder, ≥500 units/mg protein

别名:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Grade VIII

品質等級

形狀

lyophilized powder

比活性

≥500 units/mg protein

分子量

465 kDa

不包含

BSA as extender

成份

Protein, ≥60% biuret

儲存溫度

−20°C

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相关类别

一般說明

β-半乳糖苷酶是一个四聚体,由四个相同的亚基组成,每个亚基的分子量都为135,000。它是一种含巯基的酶,每个亚基含有19个半胱氨酸残基。

應用

β-半乳糖苷酶可与特异性识别靶分子的抗体偶联(酶免疫测定或EISA)。β-半乳糖苷酶也可用作报告酶以监测启动子的基因表达水平。它可以用作具有抗β-半乳糖苷酶抗体的阳性对照蛋白。

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

物理性質

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

單位定義

在37 °C,pH 7.3条件下,一个单位每分钟可将1.0μmole邻硝基苯基β-D-半乳糖苷水解为邻硝基苯酚和D-半乳糖。

外觀

含有Tris缓冲盐和氯化镁

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Alan Merk et al.
IUCrJ, 7(Pt 4), 639-643 (2020-07-23)
We report the determination of the structure of Escherichia coli β-galactosidase at a resolution of ∼1.8 Å using data collected on a 200 kV CRYO ARM microscope equipped with a K3 direct electron detector. The data were collected in a single 24 h
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
Assays for bacterial mucin-desulfating sulfatases.
A M Roberton et al.
Methods in molecular biology (Clifton, N.J.), 125, 417-426 (2000-05-23)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
Toshiya Suzuki et al.
Plant physiology, 173(1), 183-205 (2016-11-12)
Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we

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